Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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The analysis directly from clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. Primers designed for the zujeszky amplification of the viral gD glycoprotein gene of the PRV genome. Finally, nucleic acids from tissue homogenates samples derived from seven healthy pigs, and a non infected PK cell line were also tested showing no positive products data not shown.
The virus principally affects suinod, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8. The analytical sensitivity of the test was consistently observed to be 1.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
The PCR for PRV genome detection is also an important method in screening pig specimens collected for xenotransplantation to increase the safety of organ dr 7 and to detect viral infection in a wide spectrum of species reported to be susceptible to PRV, through either natural or experimental infections 8. Induction and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion during acute infection of swine.
Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis.
Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed.
Journal List Braz J Microbiol v. Cell ee and molecular characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract. The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome This region was highly conserved for all reported genomes as shown by aligning of these sequences.
Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier. Iowa State University Press; The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals.
Open in a separate window. The possibility to perform annealing and elongation in one single step of the thermal profile contributed to the specificity and the efficiency of the assay and allowed the use of a very fast PCR program. The polymerase chain reaction PCR can be used to identify PRV genomes in secretions or organ samples and although some PCR assays for Dofna detection with different sensitivities have been reported 37915 there is no standard procedure recommended so far 2.
A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided.
Seven virus-negative tissues samples from clinically healthy animals were also included. The trigeminal ganglion is the most consistent site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site. This paper describes the development, optimization and performance assessment of a rapid and suinod sensitive PCR test for detection of pseudorabies virus.
The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent dena. Can J Com Med. Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders Author information Article notes Copyright and License foena Disclaimer. A rapid and accurate diagnosis of PRV infection is important for the initiation of appropriate control strategies.
The viral agent following a primary replication can establish latent infection and develops a latency-reactivation infection which allows its perpetuation in pig populations 1012 National Center for Biotechnology InformationU. Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments.
The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected. PRV specific primers were designed using the Oligo 6. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs.
Doença de Aujeszky
This assay was based on the amplification of a highly conserved viral gD gene fragment. Since the rapid detection of infected animals would reduce the potential transmission of the viruses to uninfected herds avoiding the spread of the diseases In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders.
The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine. Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs However, this method is time-consuming and false negative results may occur in submissions from latently infected animals The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction.
Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2. Primers sequences, genome positions and the size of PCR products are shown in Table 1. Potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine.
Published online Sep 1. The Sma I restriction endonuclease site was used for additional specificity confirmation of the amplification products.